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Fig. 6 A vital role for S18-2 during zebrafish embryogenesis. (A) The mRNA expression levels of RB1 (blue line) and S18-2 (red line) were assessed at different stages of zebrafish development using qPCR. The mean values of three qPCR experiments are shown. The SD was not more than 20% of the mean. β-Actin was used as a housekeeping gene, and the expression levels detected in eggs at 0 hpf were taken as the reference expression level. (B) A specific morpholino that inhibited S18-2 translation, a control morpholino, or a S18-2–specific morpholino, together with in vitro-translated S18-2 protein, were introduced into fish eggs within 1 hpf. The morphology of the larvae was observed over 1 to 72 hpf using bright-field microscopy. (C) Quantification of embryos showing morphological changes at 24 h (see details in SI Appendix, Table S3) after the indicated treatments. (D) Quantification of embryos showing morphological changes at 48 h after the indicated treatments. Each bar in C and D represents the mean of four to five measurements; the deviation was less than 30%.