ZFIN Image: Cang et al., 2020, Fig. 6

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Figure Caption

Fig. 6 Application of SpaOTsc to spatial transcriptomic data and their integrations with scRNA-seq datasets.

The Slide-seq data, the RNA seqFISH+ data, and the scRNA-seq data for mouse olfactory bulb were taken from ref. ⁵¹, ref. ⁵², and ref. ⁵³, respectively. a, b Spatial distributions of signal senders and receivers with the color showing the likelihood of being a sender or receiver. Top row: inference using only spatial transcriptomics data. Bottom row: the inferred signaling in scRNA-seq data (sample WT1) visualized by mapping the single cells to space using spatial transcriptomic data. c Signaling for four individual marked ligands in the Slide-seq data. d The intercellular gene–gene regulatory information flow of the top 20 variable genes in the Slide-seq data. e Similarities on cluster–cluster communication between the six samples of the scRNA-seq data integrated with the RNA seqFISH+ data measured by Pearson’s correlation coefficient.

Acknowledgments

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