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Fig. 11 sav1 and stk3 display a synergistic genetic interaction. (A-D′) Toluidine Blue stained Epon sections of liver and gallbladder in wild-type, sav1−/−, stk3−/− and sav1−/−;stk3−/− larvae at 7 and 10 dpf. Gb, gallbladder. Red dashed lines outline the liver. Scale bars: 100 μm. (E) Ratiometric analysis at 8 dpf of BODIPY FL C5 gallbladder uptake in larvae from a sav1+/−;stk3+/− incross. (F) TEM of wild-type, sav1−/− and sav1−/−;stk3−/− Epon sections at 10 dpf reveals the most severe phenotype in sav1−/−;stk3−/− larvae. (G) Toluidine Blue stained epon sections of proximal renal tubules in wild-type, sav1−/−, stk3−/− and sav1−/−;stk3−/− larvae at 10 dpf. PRT, proximal renal tubule. Only double mutants display a dysplastic and fibrotic phenotype. (H) TEM of larvae from G.