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Fig. s1 Dusp6 expression in nephrogenic cell aggregates. (A) Immunostaining with anti-Dusp6 (red) co-localizes with anti-GFP immunostaining of the lhx1a:eGFP transgene (B) at 7 dpi. (C) Merged image of A and B. Dotted line represents outline of adjacent distal tubule. Scale bars are 10 μm. (D) Injury-induced expression of dusp6 is reduced by PD166866 (added to tank water from 1 dpi to 7 dpi) to sham injured levels. qPCR represents N = 4 fish per condition. All comparisons not significant by Kruskal-Wallis test.
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Reprinted from Developmental Biology, 454(1), Gallegos, T.F., Kamei, C.N., Rohly, M., Drummond, I.A., Fibroblast growth factor signaling mediates progenitor cell aggregation and nephron regeneration in the adult zebrafish kidney, 44-51, Copyright (2019) with permission from Elsevier. Full text @ Dev. Biol.