|
Fig. 3 S1
Cardiomyocyte proliferation does not appear to be affected by modulation of glycolysis.
(a) Confocal images (mid-sagittal sections) of 131 hpf Tg(myl7:EGFP-HRAS) alone or in combination with Tg(myl7:pdha1aSTA-P2A-tdTomato) and of 131 hpf Tg(myl7:EGFP-HRAS); pkma2-/-; pkmb-/- hearts; magnified view of area in white boxes shown below; arrowheads point to trabecular CMs; percentage of trabecular area shown on the right (n = 5 ventricles). (b) Confocal images (mid-sagittal sections) of 77 hpf Tg(myl7:mVenus-gmnn); Tg(myl7:BFP-CAAX) alone or in combination with Tg(myl7:pdha1aSTA-P2A-tdTomato) or Tg(myl7:pdk3b-P2A-tdTomato) hearts; arrowheads point to myl7:mVenus-Gmnn+ CMs; percentage of myl7:mVenus-Gmnn + CMs shown on the right (n = 5 ventricles). Error bars, s.e.m.; *p<0.05 and **p<0.001 by two-tailed unpaired t-test (a) or ANOVA followed by Tukey’s HSD test (b). NS, not significant. Scale bars, 20 μm.