Fig. 9

Fig. 9

a IF staining of FLAG and β-catenin showing the co-localization of FLAG-Adnpa and β-catenin in 3.5 hpf adnpa−/− adnpb−/− embryos injected with mRNAs encoding FLAG-Adnpa. FLAG-Adnpa is predominantly expressed in the nucleus. b IF staining of FLAG and β-catenin showing the co-localization of FLAG-Adnpa and β-catenin in 6 hpf adnpa−/− adnpb−/− embryos injected with mRNAs encoding FLAG-Adnpa. FLAG-Adnpa is predominantly expressed in the nucleus. c Co-IP data showing FLAG-Adnpa interact with β-catenin in 24 hpf adnpa−/− adnpb−/− embryos injected with mRNAs encoding FLAG-Adnpa. d IF staining of β-catenin and HuC showing slightly reduced HuC and β-catenin levels in 16 hpf adnpa−/− adnpb−/− (adnpab−/−) embryos. e IF staining of β-catenin and HuC showing significantly reduced HuC and β-catenin levels in 24 hpf adnpa−/− adnpb−/− embryos. f WB data showing slightly reduced β-catenin levels in 24 hpf adnpa−/− adnpb−/− embryos. g TopFlash luciferase activity assay for total lysates made from 24 hpf control, double mutant embryos and double mutant embryos injected with adnpa mRNAs. Three pools of ten embryos each (n = 3 per group) were lysed with the passive lysis buffer and assayed for luciferase activity. Data are based on two biologically independent experiments, and similar results were obtained. h Representative morphology of 2 dpf adnpa overexpressing embryos. The dorsalized phenotypes (C1–C4) were according to the DV patterning index³⁸. All experiments were repeated at least two times, and similar results were obtained. Shown are representative images. Scale bars: 50 μm in (a, b, d), 100 μm in (e), and 200 μm in (h).