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Figure 3—figure supplement 1.

ID: ZDB-IMAGE-200530-42
Source: Figures for Ye et al., 2020

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Figure Caption

Figure 3—figure supplement 1. Create <italic>lin28a</italic> mutant using CRISPR.

(A) Diagram shows four exons of lin28a and the position of CIRPSR that was used for creating lin28a mutant. (B–C) Deletion of five nucleotides in the lin28a mutant allele damages the restriction enzyme site of Hpy188I and leads to a truncated protein by creating a premature stop codon in lin28a mRNA. (D) F1 embryos were genotyped for lin28a mutation by PCR followed by Hyp188I digestion. (E) Transcribed lin28a mRNA level was decreased in lin28a-/- post LY+neo, probably because the mutant form of lin28a mRNA is unstable and degraded.

Acknowledgments

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