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Fig. 7 Urp over-expression causes dorsal curvature of the body axis in zebrafish embryos. (A) Wild-type embryos injected with hs::urp2 transgene, without heat induction. Note no effect on the body axis. (B) Same batch of embryos imaged after 30 min post-heat shock. Note the initiation of dorsal curvature of the body axis in the heat-shocked embryos (arrows). (C) A wild-type embryo at 72 hpf injected with myog::urp2 transgene, showing dorsal curvature of the trunk and tail (arrow). (D) A wild-type embryo at 5 dpf injected with myog::urp2 transgene showing spiral coiling of the body axis (arrow). Embryos shown in A–D are representative of a minimum of 100 embryos analysed for each condition of Urp2 over-expression, in two independent experiments. (E) A wild-type embryo at 48 hpf, injected with water in the dorsal somite, imaged immediately after injection. (F) The same wild-type embryo depicted in E, imaged after 1 h. Note no effect on body axis position. (G) A wild-type embryo at 48 hpf, injected with synthetic Urp1 in the dorsal somite, imaged immediately after injection. (H) The same wild-type embryo depicted in G, imaged after 5 min. Note the dorsal curvature of the body axis (arrow). In E and G, the injection sites are indicated (magenta arrows). Four embryos were injected for each condition. Scale bars: 1 mm.