Fig. s3 Neural crest markers, dlx2a and crestin, are unchanged in the pharyngeal arches with loss of prdm3 in zebrafish. (A-B) Wildtype or prdm3−/− mutant embryos were collected and in situ hybridization was performed for dlx2a (A) or crestin (B) at 24 hpf. Shown are lateral views of dlx2a expression in the pharyngeal arches (1–4) (A) and lateral views of crestin across the whole body to show neural crest migration is unaffected in prdm3 mutant zebrafish. Higher magnification, dorsal views of crestin expression is also shown in (B). Scale bars, 100 μm. Abbreviations: eye (e), 1–4 (pharyngeal arches 1–4), posterior pharyngeal arches (ppa).
Reprinted from Developmental Biology, 461(2), Shull, L.C., Sen, R., Menzel, J., Goyama, S., Kurokawa, M., Artinger, K.B., The conserved and divergent roles of Prdm3 and Prdm16 in zebrafish and mouse craniofacial development, 132-144, Copyright (2020) with permission from Elsevier. Full text @ Dev. Biol.