Wavelengths used in electrophysiological recordings and photocurrent amplitude and kinetics as a function of irradiance.
(A) LED emission wavelength (centre/bandwidth, nm) and irradiance levels used for each opsin line and control cells. (B) Number of cells patched in each group. Numbers and coloured bars indicate included cells while grey bars indicate excluded cells (see Materials and methods for inclusion criteria). (C,D) Access resistance (C) and cell capacitance (D) were comparable between groups (mean ± SD, across cells). (E) Example photocurrents from a CoChR-expressing cell at different irradiance levels (3–20 mW/mm2). F–H Peak photocurrent amplitude (F), activation time (G) and deactivation time constant (H) vs. irradiance (mean ± SEM, across cells). Dotted lines in (F) show range of pMN rheobase. Asterisks indicate a significant non-zero slope.
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