Fig. 5

Fig. 5 Exogenous FGF beads are sufficient to recruit progenitor cells. A) Stem cells marked by the transgene lhx1a:eGFP are not attracted to BSA soaked agarose beads after 24 h of culture. (B) Beads soaked with Fgf4 are more closely surrounded by renal stem cells, indicated by a decrease in cell to bead distance from an average of 28.8 μm–17.75 μm. (C) Beads soaked with FGF10 strongly attract renal stem cells which accumulate adjacent to the bead at a mean distance of 3.42 μm. (D) Quantification of cell to bead distances after 24 h of culture demonstrate a chemoattractive role of FGF ligands on the recruitment renal stem cells. 3 biological replicates per condition. Error bars indicate ±SD. Comparisons to BSA beads were made by Kruskal-Wallis test. ns indicates not-significant, starred comparison indicates p = 0.008. In Z-projection panels (A–C) scale bar is 25 μm.