Fig. 1

Fig. 1 Human Cancers Have Similar Growth Kinetics, Histology, and Rates of Proliferation and Apoptosis when Engrafted into prkdc−/−, il2rga−/− Zebrafish or NSG Mice Human cancers have similar growth kinetics, histology, and rates of proliferation and apoptosis when engrafted into prkdc−/−, il2rga−/− zebrafish or NSG mice. Engraftment of EGFP+ human tumor cells into prkdc−/−, il2rga−/− zebrafish grown at 37°C (A–F) or the same tumors grown in NSG mice for 28 days (G–L). Merged fluorescence and brightfield images of whole zebrafish imaged at 0 and 28 days post transplant (dpt; A) and compared with images of engrafted mice (G, left panels) and excised tumors (G, right panels). Hematoxylin and eosin stained sections (zebrafish, B; mouse, H) and immunohistochemistry for cell lineage markers (zebrafish, C; mouse, I), Ki67 (zebrafish, D; mouse, J) and TUNEL (zebrafish, E; mouse, K) with the average percentage of positive cells ± standard deviation noted (n ≥ 3 animals/tumor type). Quantification of relative tumor growth in engrafted prkdc−/−, il2rga−/− zebrafish (F) and NSG mice (L). Human embryonal rhabdomyosarcoma RD (ERMS RD), alveolar rhabdomyosarcoma Rh41 (ARMS Rh41), melanoma UACC62, and triple-negative breast cancer MDA-MB-231. Scale bars represent 0.25 cm (A); 50 μm (B–E, H–K); and 0.5 cm (G). See also Table S1 and Figure S1.