ZFIN Image: Ren et al., 2020, Fig. 6

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Figure Caption

Fig. 6 Overexpression of <italic>igf2bp3</italic> leads to enhanced maternal mRNA stability and causes developmental delay in wild-type embryos.

a Time-matched bright field images of embryos indicated that igf2bp3-overexpressing embryos experienced a developmental delay at early stages. Scale bars: 200 μm. b The percentage of embryos with developmental delay at shield stage. The embryos exhibited delayed development in non-injected groups (N1 = 3/572, N2 = 7/515, N3 = 19/622), in GFP mRNA injected groups (N1 = 12/411, N2 = 4/460, N3 = 16/487), and in igf2bp3 mRNA injected groups (N1 = 378/481, N2 = 412/514, N3 = 439/503). c qRT-PCR analysis of maternal mRNA decay in wild type and igf2bp3-overexpressing embryos. mRNA abundance was normalized to values of wild type. Error bars represent mean±S.D., n = 3. P values were calculated by two-sided Student’s t-test (*p < 0.05, **p < 0.01, ***p < 0.001).

Acknowledgments

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