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Fig. 4-S1
Maternal zygotic embryos completely lack inflated vacuoles.
( A,C,E) DIC images of 24 hpf spzl+/-, zygotic spzl-/-, and maternal zygotic spzl-/- embryos respectively. ( B,D,F) Live confocal images of 24 hpf spzl+/-, zygotic spzl-/-, and maternal zygotic spzl-/-embryos respectively expressing SAG:gal4;UAS:GFP in the notochord. Asterisks indicate inflated vacuoles. Arrows point to areas lacking fully inflated vacuoles.
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