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Fig. 2 RPIA-transgenic zebrafish develop steatosis, hyperplasia, dysplasia and HCC at earlier stages than control transgenic zebrafish. (A) Representative images of H&E staining from livers of controls and the two transgenic RPIA lines between 3- and 11-month-old fish. All of the magnifications were ×400. Scale bar: 50 μm. (B) Statistical analysis of H&E stain from 3 to 11 months of control fish (mCherry) and two lines of RPIA-transgenic fish (Tg1 and Tg2). N = 10 for each stage. Tg1 developed HCC as early as 9-month old, while Tg2 developed HCC as early as 7-month old.
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