ZFIN Image: Hoelscher et al., 2020, Figure 6

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Figure 6

Role of miR-128a in cardiac development. During murine in vitro differentiation, LNA-mediated miR-128a knockdown in differentiating ES/iPSCs (1) increased cardiac transcription factors such as Isl1, Sfrp5, and Hcn4 but reduced Irx4 at the onset of cardiogenesis; (2) upregulated Isl1-positive CPCs, whereas NkxCE-GFP-positive CPCs were downregulated; and (3) increased the expression of the ventricular cardiomyocyte marker Myl2 accompanied by a reduced beating frequency of early cardiomyocytes. Overexpression of miR-128a (4) diminished the expression of Isl1, Sfrp5, Nkx2.5, and Mef2c, but increased Irx4, (5) enhanced the NkxCE-GFP-positive CPC population, and (6) favored nodal-type-like cardiomyocytes marked by Tnnt2, Myh6, and Shox2 accompanied by increased beating rates. Parts of the figure from BioRender.com

Acknowledgments

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