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Fig. 5 Laser Ablation-Induced NCC Phagocytosis (A) Images from a time-lapse movie of a Tg(sox10:Gal4);Tg(UAS:GFP-FYVE) embryo after ablation of a NCC (outlined in yellow) at 24 hpf. Arrowheads denote NCC engulfment vesicles. Cyan and red arrowheads denote newly formed and pre-existing vesicles, respectively, in each frame, correlating with the quantification in (B). A NCC that engulfed twice is outlined in cyan. (B) Quantification of phagosome formation in (A). Top panel shows phagosome count per frame fitted with a Gaussian curve (R square = 0.7075). Bottom panel shows the duration of each PI(3)P+ vesicle. (C) Quantification of the number of times NCCs phagocytose in a 100 μm2 region around the ablation site in 100 mpa (mean ± SD, n = 5 fish). (D and E) Quantification of the number of NCC phagosomes per frame before and after cell ablation (D), or distal and proximal to ablation sites (E) (n = 50 frames). mpa, minutes post ablation. Scale bar, 10 μm.
Reprinted from Cell, 179(1), Zhu, Y., Crowley, S.C., Latimer, A.J., Lewis, G.M., Nash, R., Kucenas, S., Migratory Neural Crest Cells Phagocytose Dead Cells in the Developing Nervous System, 74-89.e10, Copyright (2019) with permission from Elsevier. Full text @ Cell