ZFIN Image: Pang et al., 2020, Figure 1

Image

Figure Caption

Figure 1 Generation of the mutant <italic>dlx3b</italic> zebrafish line.

(A) Structure of the DLX3 and dlx3b proteins in human and zebrafish. The homeodomain and transactivation domains are marked in yellow and blue, respectively. The green arrows indicate the TDO mutation sites that found in patients. The red arrow indicates the TALEN target site in zebrafish. (B) Sequence of the TALEN target site and mutant site in zebrafish dlx3b. TALEN binding sites in the dlx3b gene are indicated in gray shading, and the HindIII restriction site is highlighted in red. The sequence of mutant dlx3b mRNA is aligned with wild-type dlx3b mRNA. (C) Predicted peptide sequences for the wild-type and mutant dlx3b. There is a frameshift mutation at 182 aa, and the subsequent mutant sequence is highlighted in red.

Acknowledgments

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