Fig. S4

Fig. S4

Morphology of the cerebellum and the mesencephalic tectum in reln mutants. Cross sections of the cerebellum (A-D) and the mesencephalon (E-H) of adult WT (A, E, G, n= 2) and relnΔ7/Δ7 mutant (B, C, D, F, and H, n= 2) fish were stained by the Bodian silver impregnation method, which visualizes neuronal fibers (gray) and cell nuclei (purple). (C, D) High magnification images of the relnΔ7/Δ7 mutant cerebellum. Arrows indicate ectopic neurons that have a large soma and are probably PCs in the GCL. (G, H) High magnification images of the WT and relnΔ7/Δ7 mutant tectum. Note that no gross abnormalities in the tectum of the reln mutant were observed. Scale bars: 100 μm in A (applies to A, B); C (applies to C, D); E (applies to E, F); and G (applies to G, H).