ZFIN Image: Son et al., 2020, Figure 4.

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Figure Caption

Figure 4.

Hypoxia causes a decrease in DDT/motor neuron synapses. A, Confocal images of spinal cord of Tg(otpb.A.:TRFP-caax); Tg(Hb9:FingR(PSD95)-GFP) larvae, anti-GFP, anti-RFP labeling. Rostral to top, DDT labeled in red, motor neurons in green, synapses in yellow. B, Magnified view of IMARIS imaging of synapses. C, Quantification, mean and SD, **p < 0.01. Number of motor neurons and intensity of DDT is not affected by hypoxia, but number of synapses is decreased, including when corrected for motor neuron number. D, Confocal images of spinal cord of Tg(Hb9:FingR(PSD95)-GFP) larvae with anti-TH anti-GFP immunohistochemistry. Right panels, IMARIS panel image shown to right of corresponding confocal image. E, Quantification with mean and SD shows that number of motor neurons and intensity of DDT is not affected by hypoxia, but number of synapses is decreased, including when corrected for motor neuron number. *p < 0.05.

Acknowledgments

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