Figure 1.

Figure 1.

Dopaminergic synapses to spinal cord motor neurons. A, Confocal images, lateral views of zebrafish spinal cord at 5 dpf, dorsal to top; scale bar: 10 µm. DAPI staining of cell nuclei; anti-Hb9 immunohistochemistry in motor neurons; anti-TH immunohistochemistry of DDT; and double-labeling anti-Hb9 anti-TH shows co-localization (arrows). B, Confocal images, dorsal views of zebrafish at 5 dpf, rostral top. Left panel shows entire larva with DDT labeled by GFP in Tg(otpb.A:EGFP-caax) line. Higher magnification (scale bar: 50 µm) shows DAPI staining of cell nuclei; GFP labeling of DDT; anti-TH labeling of TH; and co-localization of TH and GFP in DDT. C, Tg(otpb.A:TRFP-caax); Tg(Hb9:FingR(PSD95)-GFP) larvae, confocal images, dorsal views, of spinal cord at 5 dpf (scale bar: 10 µm). FingR localization of PSD95, expressed in motor neurons, of synapses can be seen neighboring DDT labeled by RFP (arrowheads). D, Anti-D4R labeling co-localizes with FingR GFP expression (scale bar: 10 µm).