Figure 2

Quantification of ISV lumen diameter in (B), the numbers of defective ISVs in (C), the numbers of embryos with aberrant DLAVs in (D), and the numbers of ruptured DLAVs in (E) from data observed in equivalent fields of view (within eight somites). The severity of blood vessel defects in cep41‐deficient zebrafish: B (narrowed ISVs) < C (shorten, fused, and missing ISVs) < D and E (ruptured DLAVs). Data are shown as mean ± SD of three independent experiments with ≥ 20 embryos per condition. Statistical significance was determined using the one‐way ANOVA followed by Dunnett's post hoc test (B) and the Kruskal–Wallis test by Dunn's post hoc test (C, E) (**P < 0.01, ***P < 0.001).

CV protrusions (arrowheads) and vascular loops (asterisks) were observed in zebrafish at 35 hpf. The images within the dotted rectangles are magnified in the right panels. A, anterior; P, posterior; CA, caudal artery; CV, caudal vein. Scale bars, 40 μm. Quantification of the length of the CV protrusions in (G) presented graphically by measuring them in equivalent fields of view (within five somites). Data are median of four independent experiments (n ≥ 5 embryos per experimental condition). Statistical significance was determined using the Kruskal–Wallis test followed by Dunn's post hoc test (***P < 0.001, ns: non‐significant).