Figure 2.

Figure 2.

In the mdka^mi5001 mutant, Müller glia fail to proliferate in response to photoreceptor cell death. A, Immunocytochemistry for PCNA (magenta) in WT and mdka^mi5001 at 1 and 3 dpl. In WT, Müller glia become positive for PCNA at 1 dpl. At 3 dpl, PCNA⁺ progenitors form neurogenic clusters. Mutant retinas lack PCNA-labeled cells at 1 dpl. Very few, isolated cells are positive for PCNA in the mdka^mi5001 at 3 dpl. B, C, The number of PCNA⁺ cells in WT and mdka^mi5001 in the inner (B; 1 dpl: p = 0.0017; 2 dpl: p < 0.0001; 3 dpl: p < 0.0004, ANOVA with post hoc Tukey; F ratio = 116.1834) and outer (C; 3 dpl; p = 0.0001, ANOVA with post hoc Tukey; F ratio = 17.5589) nuclear layers. The mdka^mi5001 retinas have significantly less PCNA⁺ cells at 1, 2, and 3 dpl compared with WT retinas. A total of 3 sections were counted and averaged in each retina. A total of 3 retinas were analyzed. Scale bar, 30 μm. *p < 0.01.