Figure 6

Figure 6

LPA₃ protects fibroblasts against cell senescence caused by oxidative stress. (a) CCK‐8 assay showed that knockdown of LPA receptors alone had no effect on cell proliferation. (b) CCK‐8 revealed that treatment with 10 μM and 50 μM H₂O₂ for 3 days significantly decreased cell proliferation in knockdown of LPA₃, but not of LPA₁ or LPA₂. CCK‐8 reads were normalized to a sham group. (c) Representative images of senescence‐associated β‐gal staining assay. Treatment with 10 μM and 50 μM H₂O₂ for 3 days significantly increased percentages of β‐gal + cells in knockdown of LPA₃, but not of LPA₁ or LPA₂. Quantification was shown in (d). (e) Western blot showed that 100 μM of H₂O₂ treatment for 6 hr increased protein level of Nrf2, NQO1, SOD2, and Gpx1, whereas LPA₃ knockdown abolished the effects. (f) Graphical abstract of this study. ANOVA and Student's t test; *p < .05, **p < .01, ***p < .001