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Figure 5 E-cadherin regulates localisation of aPKC and Lgl2 in the epidermis. Confocal images showing GFP marked clones (A1, B1, C1) marking cadherin-1 morpholino (cdh-1 MO) or control MO (Ctrl MO) and immunolocalisation of E-cadherin (A2, B2, C2), aPKC (A3, A4) and Lgl2 (B3, B4, C3, C4). Arrowhead marks the loss of E-cadherin in morphant cells (A2, B2, C2). Different z-stacks depicting apical (A3) or basal domains (A4) of peridermal cells showing aPKC immunostaining. Arrow shows enrichment of aPKC in the basal domain of the cdh-1 morphant cells (A4). Intensity profiles for aPKC in the basal domain in non-clone (NC) cells (A5) and clone (C) cells (A6) were measured along the dotted arrows (in A4). Lgl staining in the periderm (B3, C4) or basal epidermis (B4, C3). Arrows show the high levels of Lgl in the morphant or juxtaposed cells (B3, B4, C3, and C4) upon e-cadherin knockdown. Graphs showing levels of Lgl across normalised cell height in the periderm (B5, C6) and basal epidermis (B6, C5) across different boundaries mentioned. Dotted line indicates the position of the clone in the periderm (B1–B3) or basal epidermis (C1–C3) and corresponding region in basal epidermis (B4) or periderm (C4), respectively. Scale bar represents 10 µm in (A4, B4, C4). AU = Arbitrary Units. Source file with fluorescence intensities for peridermal clone and boundary analysis in periderm (B5) and basal epidermis (B6) is available as Figure 5—source data 1 and 2, respectively. Fluorescence intensities for basal epidermis clone and analysis of boundaries in basal epidermis (C5) and periderm (C6) is available as Figure 5—source data 3 and 4, respectively. Statistical analysis of intensity comparisons along with p values is available as Figure 5—source data 5.