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Figure 2 Immunolocalisation of E-cadherin along the apicobasal axis (0 µm is apical) in wild-type (WT) sibling and has/apkc mutant in the periderm (A1) and basal epidermis (B1) at 48hpf. Comparison of height of cells (A2, B2) and apical perimeter (A3, B3) in the periderm (A2, A3) and basal epidermis (B2, B3) of the two genetic conditions. Graphs showing polarised localisation of E-cadherin across normalised cell height in the periderm (A4) and basal epidermis (B4). A graph showing noise index in E-cadherin localisation in WT siblings versus has/apkc mutants (A5). Arrows in A4 pointing to the abnormal localisation of E-cadherin. Scale bars in A1, B1 are equivalent to 10 µm. AU = Arbitrary Units; WT = wild type, sib = sibling/s and has/apkc = has/apkc mutants. Asterisk indicates significant difference at p<0.05 and n.s. means non-significant difference observed by Mann Whitney U test. Source file with fluorescence intensities for periderm and basal epidermis is available as Figure 2—source data 1 and 2, respectively. Statistical analysis with p values for all the graphs of periderm and basal epidermis is available as Figure 2—source data 3 and 4, respectively.