ZFIN Image: Hughes et al., 2019, Fig. 1

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Figure Caption

Fig. 1

Axons accumulate synaptic vesicle release machinery under myelin sheaths. a Schematic of transgenes used to assess synaptic puncta density along myelinated and bare regions of axons. b Representative image of Syp-eGFP in a myelinated phox2b + axon in a living 3 dpf Tg(phox2b:GAL4; UAS:syp-eGFP; sox10:mRFP) larva. Bracketed dashed lines indicate two myelin sheaths (rectangles) on the axon. b′ Same as (b) for Vamp2-eGFP; animal genotype is Tg(phox2b:GAL4; sox10:mRFP) with transient expression of UAS:Vamp2-eGFP. c Representative images of Syp-eGFP distribution in myelinated phox2b + axons at 3, 4, and 5 dpf. d Comparisons of Syp-eGFP puncta density within bare vs myelinated regions of axons at each timepoint. n = 7 bare regions/98 puncta and 7 sheaths/18 puncta (3 dpf); 8 bare/119 puncta and 11 sheaths/43 puncta (4 dpf); 9 bare/75 puncta, and 12 sheaths/38 puncta (5 dpf). Wilcox rank-sum test, p = 0.13, p = 0.002, p = 0.00025 for 3, 4, and 5 dpf, respectively. Scale bars, 10 µm

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Expression / Labeling details

Acknowledgments

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