ZFIN Image: Xu et al., 2019, Figure 3

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Figure Caption

Figure 3

Expression of genes related to body axis formation, autophagy, and apoptosis. (A) WISH of col2a1a, col8a1a, and shha in embryos at 24–28 hpf. (B) Expression of autophagy- and apoptosis-related genes in embryos at 24–28 hpf, detected using qRT-PCR. β-actin was used as an internal control. (C) WISH of cyclinD1, pax2a, opl, and neuroD1, which are involved in neural system development. (D) Gray-scale analysis of WISH results of genes in (C), whose signal strength was measured using ImageJ. (E) Expression of cyclinD1, pax2a, opl, and neuroD1 in embryos, detected using qRT-PCR, was normalized using β-actin.

Acknowledgments

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