ZFIN Image: Xu et al., 2019, Figure 2

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Figure Caption

Figure 2

Knockdown of Drfundc1 caused abnormal body axis development in zebrafish embryos (A,B), which was repaired by Drfundc1 mRNA (C–E). (A) Midline bifurcation and headless embryos caused by microinjection of Drfudnc1 shRNA1 or shRNA2 into zygotes at 24–28 hpf. (B) shRNA2 significantly decreased endogenous Drfundc1 in the embryos (***P < 0.001), as measured by qRT-PCR with normalization by β-actin. (C) Co-injection of Drfundc1 mRNA repaired defects caused by shRNA2. Before injection, 200 pg/nL of shRNA2 was mixed with 400 pg/nL of mRNA. Control embryos were not injected. (D) WISH results of Drfundc1 in embryos with different treatments. (E) Gray-scale analysis of WISH results of Drfundc1 (D), measured using ImageJ. Letters a, b, and c indicate significance (P < 0.05). All embryos were oriented with a dorsal view. Arrows point to abnormal sites in the embryos.

Acknowledgments

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