ZFIN Image: Sharma et al., 2019, Figure 4.

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Figure Caption

Figure 4. Involvement of Snails, Zebs, <italic>miR200a/miR-200b/miR-143/miR-145</italic>, and Cdh1 to ensure adequate number of MGPCs during retina regeneration.

(A, B) IF confocal microscopy images of 4 dpi retinal cross sections show BrdU⁺ MGPCs in snai1a, snai1b, snai2, and snai3 mRNA-transfected conditions along with gfp mRNA-transfected control retina (A), which are quantified (B); *P < 0.002 (t test), N = 4. (C, D) IF confocal microscopy images of 4 dpi retinal cross sections show BrdU⁺ MGPCs in zeb1a mRNA transfected conditions along with gfp mRNA transfected control retina, with control MO and oct4 MO-electroporated conditions (C), which are quantified (D); *P < 0.001 (t test), N = 4. (E, F) IF confocal microscopy images of 4 dpi retinal cross sections show BrdU⁺ MGPCs in miR-200a/miR-200b MO-electroporated conditions along with control MO (E), which are quantified (F); *P < 0.004 (t test), N = 4. (G, H) IF confocal microscopy images of 4 dpi retinal cross sections show BrdU⁺ MGPCs in miR-143/miR-145 MO-electroporated conditions along with control MO (G), which are quantified (H); *P < 0.01 (t test), N = 4. (I, J) IF confocal microscopy images of 4 dpi retinal cross sections show BrdU⁺ MGPCs in cdh1 MO-electroporated conditions along with control MO and oct4 MO (I), which are quantified (J); *P < 0.003 (t test), N = 4. Ctl MO is control MO. Error bars are SD. (A, C, E, G, I) Scale bars, 10 μm; the asterisk marks the injury site; GCL, ganglion cell layer; INL, inner nuclear layer; ONL, outer nuclear layer (A, C, E, G, I).

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Phenotype details

Acknowledgments

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