ZFIN Image: O'Brown et al., 2019, Figure 5-figure supplement 3.

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Figure Caption

Figure 5-figure supplement 3. <italic>Mfsd2aahm37/hm37; mfsd2abhm38/hm38</italic> double mutants display similar increased BBB permeability to <italic>mfsd2aahm37/hm37</italic> single mutants.

(A–D) Representative maximum intensity projection images of the midbrain of wild-type (A; mfsd2aa^+/+; mfsd2ab^+/+), mfsd2aa^hm37/hm37 single mutants (B), mfsd2ab^hm38/hm38 single mutants (C), and mfsd2aa^hm37/hm37; mfsd2ab^hm38/hm38 double mutants (D) expressing the fluorescently labeled 80 kDa transgenic serum protein DBP-EGFP (green) at 5 dpf. Mfsd2aa^hm37/hm37; mfsd2ab^hm38/hm38 double mutants display a similar level of increased parenchymal tracer permeability to the mfsd2aa^hm37/hm37 single mutants. The scale bar represents 50 µm. (E) Quantification of DBP-EGFP tracer intensity in the midbrain parenchyma of wild-type (black), mfsd2aa^hm37/hm37 mutants (magenta), mfsd2ab^hm38/hm38 mutants (green), and mfsd2aa^hm37/hm37; mfsd2ab^hm38/hm38 double mutants (turquoise). Each individual fish measured is displayed as a single point. The mean and the standard error are drawn in black. ****p<0.0001 by two-way ANOVA.

Figure Data

Phenotype details

Acknowledgments

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