ZFIN Image: Swire et al., 2019, Figure 2

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Figure Caption

Figure 2—figure supplement 2. Social isolation does not affect medial prefrontal cortex vasculature.

(A) Representative images of medial prefrontal cortex vasculature staining for PECAM1. (B) Quantification of PECAM1 area in medial prefrontal cortex layer II/III: Grouped 2.468% ± 0.3156 n = 6 mice, Isolated 2.086 ± 0.13 n = 6 mice (mean ± standard error). Mann-Whitney test. (C) Quantification of number of Pecam1 mRNA expressing cells per field of view. Grouped 9 ± 0.5888 n = 4 mice, Isolated 8.44 ± 1.162 n = 4 mice (mean ± standard error). Mann-Whitney test. (D) Quantification of the number of Pecam1 mRNA molecules. Grouped 13.47 ± 6.204 n = 77 cells from four mice, Isolated 10.89 ± 5.02 n = 92 cells from five mice (mean ± standard deviation). Mann-Whitney test, p=0.002. (E) Quantification of the number of Pecam1 mRNA molecules per mouse. Grouped 13.53 ± 1.305 n = 4 mice, Isolated 10.48 ± 1.018 n = 5 mice (mean ± standard error). Mann-Whitney test. (F) Quantification of the number of Edn1 mRNA molecules in Pecam1 positive endothelial cells. Grouped 12.08 ± 9.759 n = 77 cells from four mice, Isolated 4.837 ± 4.406 n = 92 from five mice (mean ± standard deviation). Mann-Whitney test, p<0.0001. (G) Quantification of the number of Edn3 expressing Pecam1 positive endothelial cells. Grouped 0.5463 ± 0.01586 n = 5 mice, Isolated 0.3674 ± 0.02163 n = 5 mice (mean ± standard error). Mann-Whitney test, p=0.0079.

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