Figure 2—figure supplement 2.

Figure 2—figure supplement 2.

(A, B) Brightfield view of the KV in (A) WT and (B) an embryo overexpressing Yap in DFCs at the 12-somite stage (12 s). (C, D) Visualization of the KV (green) and of KV cilia (red – immunolabeling using an anti-acetylated tubulin antibody) at the 12-somite stage in (C) WT embryos of the Tg(sox17:GFP)^s870 line and (D) embryo of the same line overexpressing Yap in DFCs. Scale bars 20 µm. (E–F) lateral view of (E) a WT embryo and (F) an embryo overexpressing Yap in the DFCs at the 18-somite stage (18 s). Arrows point to the KV. (G) Quantification of the size of the KV (expressed as the area of the planar projection of its lumen) in WT and in Yap gain of function. (H) Quantification of the number of cells present in the DFCs at early gastrula stage and in the KV at the 12-somite stage. Numerical data for (G–H) and details of statistical analysis are provided in Figure 2—figure supplements 2—source data 1.

10.7554/eLife.45241.011Figure 2—figure supplement 2—source data 1.