ZFIN Image: Johansson et al., 2019, Figure 5

Image

Figure Caption

Figure 5

Loss of Dkk1 Increases Cell Adhesion and Polarization

(A and B) Single-plane confocal images of Tg(gsc:GFP) embryos (green axis) at 80% epiboly (EB) stained with phalloidin (red). (A) Prechordal plate leading edge. A stitch-like pattern of actin distribution in cells surrounding the prechordal plate is evident in Dkk1 knock down (KD) embryos (arrowheads). (B) Filamentous actin along the anterior half of the axis. Dashed rectangles indicate the area of higher magnification of the single-plane confocal images shown on the right panels. Filamentous actin is highly concentrated in discrete puncta at the plasma membrane in the paraxial cells of Dkk1 KD embryos (arrowheads) (n = 10–15 for each condition). Scale bar, 20 μm.

(C) Single-plane confocal images of β-catenin antibody-stained Tg(gsc:GFP) embryos at the tail bud (TB) stage show strong patches of endogenous β-catenin (red) along the plasma membrane between connected cells. Dashed squares indicate the area of higher magnification shown on the right side of single-plane confocal images. Control (Ctrl) n = 5 and Dkk1 KD n = 6. Scale bar, 20 μm.

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Cell, 51(6), Johansson, M., Giger, F.A., Fielding, T., Houart, C., Dkk1 Controls Cell-Cell Interaction through Regulation of Non-nuclear β-Catenin Pools, 775-786.e3, Copyright (2019) with permission from Elsevier. Full text @ Dev. Cell