ZFIN Image: Chen et al., 2019, Figure 7

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Figure Caption

Figure 7

ROS- and mitochondrial ROS (mROS)-mediated apoptosis signaling in copper-stressed macrophages and neutrophils. ROS red labeling [(A1–A4), in red boxes] and relative ROS fluorescence (A5) in macrophages and neutrophils in copper-stressed coro1a-driven GFP transgenic larvae. (B) Schematic view for testing the mROS-mediated apoptosis gene expression in copper-stressed macrophages and neutrophils via cell direct qRT-PCR. (C) Expression of different caspase genes in macrophages and neutrophils from copper-stressed coro1a-driven GFP transgenic larvae before and after A. hydrophila infection. (D) Expression of mROS-mediated apoptotic genes in aforementioned cells. Three biological replicates were performed. ANOVA post-hoc Tukey's test. Data are presented as mean ± SD. ***P < 0.001; **P < 0.01; *P < 0.05; and ns, no significance.

Acknowledgments

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