ZFIN Image: Chen et al., 2019, Figure 6

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Figure Caption

Figure 6

Increased apoptosis of macrophages and neutrophils in copper-stressed larvae. Annexin-V [(A2–A10), in red boxes] and PI (B1–B4) labeling of apoptotic macrophages and neutrophils, and GFP-positive cells were selected for the analysis [(A1), in blue box]. FACS panels (A2,A3) and percentage of apoptotic macrophages and neutrophils (A4) in copper-stressed coro1a promotor-driven GFP transgenic larvae; FACS panels (A5,A6,A8,A9) and percentages of apoptotic neutrophils in copper-stressed mpx transgenic larvae (A7) or lyz transgenic larvae (A10). Three biological replicates were performed. ANOVA post-hoc Tukey's test. Data are presented as mean ± SD. ***P < 0.001; and ns, no significance.

Acknowledgments

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