ZFIN Image: Vetrivel et al., 2019, Fig. 12

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Figure Caption

Fig. 12

Hist2h3ca1 knock-down affects zebrafish eye development. a–c: After MO-mediated knockdown of zebrafish hist2h3ca1, FGF signaling (green EGFP reporter) was still preserved in telencephalic (te), otic vesicle (ov) and midbrain-hindbrain-boundary (mhb) regions, but lost in the lens (dashed circle) of morphant embryos (c), compared to not injected (a) and mismMO-injected controls (b), analyzed at 30 hpf (hours post-fertilization). re: retina. d–f: At 30 hpf, expression of cryba2b (red) was almost completely lost in the lens (le) of morphants (hMO) (f), compared to not injected (n.i.) (d) and mismatched (mMO) (e) controls, while isl1 expression (green) was still present in the retina (re). g–i: TGFb (TGFβ) signaling (green EGFP reporter) was activated in the brain, retina (re) and lens (le) of not injected and control-injected embryos (g, h), while it was specifically absent in the lens region (dashed circle) of morphant embryos (i) at 2 dpf (day post-fertilization). All panels display lateral views of zebrafish cephalic regions, with anterior to the left. Displayed phenotypes are representative of n = 60 embryos per condition. The scale bars are 100 μm in A and G, 50 μm in D and apply to all images in the same row.

Figure Data

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Acknowledgments

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Reprinted from Experimental Eye Research, 188, Vetrivel, S., Tiso, N., Kügler, A., Irmler, M., Horsch, M., Beckers, J., Hladik, D., Giesert, F., Gailus-Durner, V., Fuchs, H., Sabrautzki, S., German Mouse Clinic, Helmholtz Zentrum München, German Research Center for Environmental Health GmbH, Neuherberg, Germany, Adler, T., Treise, I., Busch, D.H., Aguilar-Pimentel, A., Ollert, M., Götz, A., Amarie, O.V., Stoeger, T., Schulz, H., Becker, L., Klopstock, T., Schrewe, A., Spielmann, N., Bekeredjian, R., Garrett, L., Hölter, S.M., Zimprich, A., Wurst, W., Mayer-Kuckuk, P., Hans, W., Rozman, J., Klingenspor, M., Neff, F., da Silva-Buttkus, P., Calzada-Wack, J., Rácz, I., Zimmer, A., Rathkolb, B., Wolf, E., Prehn, C., Adamski, J., Östereicher, M., Miller, G., Steinkamp, R., Lengger, C., Maier, H., Stoeger, C., Leuchtenberger, S., Gailus-Durner, V., Fuchs, H., Hrabě de Angelis, M., Hrabě de Angelis, M., Graw, J., Mutation in the mouse histone gene Hist2h3c1 leads to degeneration of the lens vesicle and severe microphthalmia, 107632, Copyright (2019) with permission from Elsevier. Full text @ Exp. Eye. Res.