ZFIN Image: Vetrivel et al., 2019, Fig. 4

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Figure Caption

Fig. 4

QPCR analysis of Histone H3.2 coding genes. a) Relative expression levels of histone genes in the wild-type tissues of brain, liver, lens and retina. Rplp0 (ribosomal protein, large, P0) was taken as the housekeeping gene, and analysis was done using the relative expression method. Values are given as fold expression levels ± SEM; n = 3 for each tissue type. The gene of interest, Hist2h3c1, is highlighted by a red box; Hist2h3c1 was found to be the most highly expressed H3.2 encoding gene in the lens. b) Gene expression changes in the embryonic tissues of Aey69 at the embryonic stages of E10.5-E12.5 using the -2ΔΔct method; the respective wild-type tissues were used as the control, and Rplp0 was taken as the housekeeping gene. Values are given as fold expression levels ± SEM. n = 3 for each embryonic stage. Statistically significant differences of the expression levels (p < 0.005) are marked by an asterisk. The mutated gene Hist2h3c1 (red box) was found to be significantly downregulated through these stages.

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Reprinted from Experimental Eye Research, 188, Vetrivel, S., Tiso, N., Kügler, A., Irmler, M., Horsch, M., Beckers, J., Hladik, D., Giesert, F., Gailus-Durner, V., Fuchs, H., Sabrautzki, S., German Mouse Clinic, Helmholtz Zentrum München, German Research Center for Environmental Health GmbH, Neuherberg, Germany, Adler, T., Treise, I., Busch, D.H., Aguilar-Pimentel, A., Ollert, M., Götz, A., Amarie, O.V., Stoeger, T., Schulz, H., Becker, L., Klopstock, T., Schrewe, A., Spielmann, N., Bekeredjian, R., Garrett, L., Hölter, S.M., Zimprich, A., Wurst, W., Mayer-Kuckuk, P., Hans, W., Rozman, J., Klingenspor, M., Neff, F., da Silva-Buttkus, P., Calzada-Wack, J., Rácz, I., Zimmer, A., Rathkolb, B., Wolf, E., Prehn, C., Adamski, J., Östereicher, M., Miller, G., Steinkamp, R., Lengger, C., Maier, H., Stoeger, C., Leuchtenberger, S., Gailus-Durner, V., Fuchs, H., Hrabě de Angelis, M., Hrabě de Angelis, M., Graw, J., Mutation in the mouse histone gene Hist2h3c1 leads to degeneration of the lens vesicle and severe microphthalmia, 107632, Copyright (2019) with permission from Elsevier. Full text @ Exp. Eye. Res.