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Fig. S4
The CDK2 inhibitor CTV313 or a pan cdk inhibitor roscovitine reduces neutrophil migration.
(A, B) Representative images (A) and quantification (B) of neutrophils recruited to infected ear in zebrafish larva treated with a cdk2 inhibitor (CTV313) or a pan-CDK inhibitor (roscovitine). Scale bar: 100 μm. (C, D) Representative images (C) and quantification (D) of neutrophils recruited to tail fin transection sites in zebrafish larva treated with CTV313 or roscovitine. Scale bar: 200 μm. (E, F) Representative images (E) and quantification (F) of total neutrophil number in zebrafish larva treated with CTV313 or roscovitine. Scale bar: 500 μm. (A-F) Assays were done with at least 3 individual founders with 3 biological repeats each containing 20 (for motility) or 25 (for neutrophil recruitment) fish per group. Result is presented as mean ± s.d., Mann–Whitney test. (G, H) Representative images (G), and quantification of velocity (H) of neutrophils in zebrafish larvae treated with CTV313 or Roscovitine. Scale bar: 100 μm. 3 embryos each from three different founders were imaged and quantification of neutrophils in one representative movie is shown, Kruskal–Wallis test.