ZFIN Image: Lekk et al., 2019, Fig. 2-S2

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Fig. 2-S2

Parapineal projections show reduced growth in sox1a^-/- mutants.

Pineal complexes in Tg(foxD3:GFP);(flh:eGFP) and sox1a^-/- Tg(foxD3:GFP);(flh:eGFP) embryos. Scale bars 25 µm. pp – parapineal. (A) Parapineal projections at 50 hpf in two typical examples of sox1a^-/- mutants and wild-type siblings. In wild-type siblings, parapineal projections exhibit extensive outgrowth in 75% of the embryos (n = 37). Parapineal projections in sox1a^-/- mutants show reduced outgrowth in all embryos (white arrows) (n = 30). (B) Parapineal projections at 4 hpf in two typical examples of sox1a^-/- mutants and wild-type siblings. In wild-type larvae, parapineal projections show extensive branching in all larvae (n = 31). Two distinct stemming points can often be observed at 4 dpf (white arrows). In sox1a^-/- mutants, parapineal projections are either stunted (85% of the larvae) or missing (15% of the larvae) (n = 41).

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Fig. 2-S2

Fig. 2-S2

Parapineal projections show reduced growth in sox1a-/- mutants.

Parapineal projections show reduced growth in sox1a^-/- mutants.