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Fig. 4
foxc1b and acta2 are co-expressed in vSMCs in the adult zebrafish brain. A) foxc1b:EGFP expressing cells are closely associated with endothelial cells (kdrl:mCherry) in the adult brain. Arrowheads point to mural cells associated with the endothelium. Arrows point to co-expression of two smooth muscle cell markers. B) acta2:EGFPexpressing smooth muscle cells are closely associated with kdrl:mCherry expressing endothelial cells along large vessels of the brain. C) foxc1b:EGFP and acta2:mCherry are partially co-expressed in smooth muscle cells on brain vessels. Scale bars represent 50 μm.
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Reprinted from Developmental Biology, 453(1), Whitesell, T.R., Chrystal, P.W., Ryu, J.R., Munsie, N., Grosse, A., French, C.R., Workentine, M.L., Li, R., Zhu, L.J., Waskiewicz, A., Lehmann, O.J., Lawson, N.D., Childs, S.J., foxc1 is required for embryonic head vascular smooth muscle differentiation in zebrafish, 34-47, Copyright (2019) with permission from Elsevier. Full text @ Dev. Biol.