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Fig. 3
The HDAC inhibitor VPA increases pancreatic endoderm at the expense of hepatic endoderm. (A) Schematic of the experimental set up. (B-G) In situ hybridization on 24 hpf embryos showing pdx1 expression. (H) Scriptaid, VPA and Alk5i treatment lead to an increase in the pdx1-positive area. (I,J) Confocal images of 32 hpf Tg(sox17:EGFP) embryos treated with DMSO (I) as control or VPA (J). L, liver; P, pancreas. (K) VPA treatment leads to an increase in pancreatic endoderm area. ns, not significant. *P≤0.05, ***P≤0.001. Error bars represent s.e.m. Scale bars: 100 µm (B); 20 µm (I).
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