ZFIN Image: Helker et al., 2019, Fig. 1

Image

Figure Caption

Fig. 1

pdx1 expression in β-cells and ductal cells. (A,A′) Visualization of Tg^BAC(pdx1:EGFP)expression. A 200 kb pdx1 BAC drives EGFP expression specifically in the pancreatic islet (arrows). Pancreatic β-cell-specific reporter signal in Tg(ins:Kaede) larva is shown for comparison. (B,B′) Confocal images of the pancreatic islet of a 120 hpf Tg^BAC(pdx1:EGFP); Tg(ins:DsRed) larva showing β-cell Tg^BAC(pdx1:EGFP) expression. (C-C‴) Confocal images of the pancreas of a 120 hpf Tg^BAC(pdx1:EGFP) larva immunostained for GFP, Pdx1 and Nkx6.1 showing colocalization of Tg^BAC(pdx1:EGFP) expression with endogenous Pdx1. (D) Dynamics of pdx1promoter activity over time as measured by Tg^BAC(pdx1:luciferase) activity. The Tg^BAC(pdx1:luciferase) signal starts to become detectable at 72 hpf, peaks at 120 hpf and decreases by 144 hpf. (E) At the peak of the Tg^BAC(pdx1:luciferase) signal, whole-body free-glucose levels start to decrease, indicating β-cell function. AU, arbitrary units. ***P≤0.001, ****P≤0.0001. Error bars represent s.e.m. Scale bars: 200 µm (A); 8 µm (B,B′); 20 µm (C).

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development