ZFIN Image: Zhu et al., 2019, Fig. S4

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Figure Caption

Fig. S4

The expression of nodal related genes in aplnra morphants

(A-B) The expression of spaw in control embryos and aplnra morphants. In control, 91.4% of embryos displayed left-sided spaw(A. a1 and B, n=93), 21.4%, 10.2% and 62.4% of embryos injected with aplnrb MO(500uM) displayed left-sided spaw, right-sided spaw and spaw disappeared (A. a2-a4 and B, n=98). (C) Compared with control embryos, at 60% epiboly, 84.8% of embryos injected with aplnra MO displayed mild decreased lft1 expression (Cc1, n=33), at 95% epiboly, 79.4% of embryos injected with aplnra MO displayed greatly decreased lft1 expression (Cc2, n=33). (D) The expression of sox17 indicated that the transcription level of sox17 was down-regulated in aplnra morphants (80.2%, n= 26), most of control embryos displayed high level of sox17 (87.5%, n=24). (E) In control, 95.7% of embryos displayed high level of vox expression (n=23), while vox was down-regulated in 78.5% of embryos injected with aplnra MO (n=28). (F) Q-PCR analysis for the nodal related genes sox32, ism1, ndnr2 and mxtx2 in control and aplnra morphants. The result indicated

that all the four genes were down-regulated in aplnra morphants.

Note: L, Left; R, Right; D, Dorsal; V, Ventral; Three repeated experiments were done for Q-PCR. *, P<0.05; **, P<0.01; NS, not significant difference.

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Acknowledgments

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