ZFIN Image: Lin et al., 2019, Fig. 2

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Figure Caption

Fig. 2

Locally Restricted mpeg1⁺ Cells Are Highly Similar to cLCs in Transcriptome and Cellular Morphology

(A) t-SNE analysis of five different cell populations isolated from the epidermis of adult fish: locally restricted mpeg1⁺ cells (n = 4), cLCs (n = 4), neutrophils (n = 3), keratinocytes (n = 3), and T cells (n = 3). Locally restricted mpeg1⁺ cells cluster together with cLCs and neutrophils.

(B) Heatmap shows relative expression of a total of 613 differentially expressed genes (|log₂FC| > 1, FDR < 0.05) among neutrophils, cLCs, and locally restricted mpeg1⁺ cells. Representative genes are indicated at the right.

(C) In situ hybridization of cldnh (red) and cd4-1 (red) in irradiated scales, followed by anti-GFP staining of locally restricted mpeg1⁺ cells (green) and cLCs (green). White arrows indicate co-localization of cldnh with locally restricted mpeg1⁺ cells, and cd4-1 with cLCs. Scale bar, 50 μm.

(D) Geimsa staining of locally restricted mpeg1⁺ cells and cLCs sorted from the epidermis of adult Tg(cldnh:GFP;mpeg1:DsRedx) fish (n = 3) and Tg(cd4-1:mCherry;mpeg1:GFP) fish (n = 3).

Acknowledgments

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Reprinted from Developmental Cell, 49(4), Lin, X., Zhou, Q., Zhao, C., Lin, G., Xu, J., Wen, Z., An Ectoderm-Derived Myeloid-like Cell Population Functions as Antigen Transporters for Langerhans Cells in Zebrafish Epidermis, 605-617.e5, Copyright (2019) with permission from Elsevier. Full text @ Dev. Cell