ZFIN Image: Elworthy et al., 2019, Fig. 7

Image

Figure Caption

Fig. 7

Endothelial cilia are present in the juvenile caudal fin vascular plexus. Immunofluorescence confocal slices of anti‐acetylated tubulin (red)‐ and anti‐GFP (green)‐stained caudal fin vascular plexus of 11‐dpf Tg(fli1a:LIFEACT‐clover)sh467^/+ (wt) (n = 15), 13‐dpf ift54^tp49; Tg(ubi:loxP‐ift54‐loxP‐myr‐mcherry,myl7:EGFP)sh488^/+;Tg(fli1a:LIFEACT‐clover)sh467^/+ (ift54−/−; ubi:ift54) (n = 4), and 13‐dpf ift54^tp49; Tg(ubi:loxP‐ift54‐loxP‐myr‐mcherry,myl7:EGFP)sh488^/+;Tg(kdrl:cre)s898^/+;Tg(fli1a:LIFEACT‐clover)sh467^/+ (ift54−/−;ubi:ift54;kdrl:cre) (n = 4). Slices at shallow and deep levels are shown along with orthogonal views resliced through the confocal stack at the level of cilia marked by arrows. Autofluorescent red blood cells (R) are visible in the vessel lumen. GFP, green fluorescent protein; WT, wild‐type. Scale bar = 10 μm

Figure Data

Expression / Labeling details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Dev. Dyn.