Fig. 4

(a) Raster plot (upper left panels) and histogram (lower left panels, 500 ms bins) of complex spikes occurring across trials during translational whole-field motion of black and white bars in all four cardinal directions for two example Purkinje cells (PC). Numbers assigned to PCs for this and panels b-c are arbitrary. (b) Raster plot (upper left panels) and histogram (lower left panels, 100 ms bins) of complex spikes occurring across trials during whole- and half-field bidirectional rotational motion of a black and white windmill for an example cell. The dashed lines over the histogram show the velocity of the stimulus in each direction across the trial. (c) Raster plot (upper left panels) and histogram (lower left panels, 100 ms bins) of complex spikes occurring across trials during whole-field light/dark flashes for two example cells, (i) and ii). (d) A box plot of complex spike firing rates during blank trials (no visual stimuli) for cells grouped by their sensory or motor complex spike category (see Figure 2). N = 31, 14, 5, 8. Asterisks indicate significance (one-way ANOVA with Bonferroni post hoc correction, p<0.001). j (i) The location of cells colored by complex spike phenotype are plotted onto a flattened dorsal view of the cerebellum with all coordinates flipped to the right half of the cerebellum. e (ii) Three example maximum projection images of traced axonal morphology from stochastically-labelled, Fyn-mClover3-expressing Purkinje cells for which electrophysiological recordings were also obtained. Labels for each cell refer to the electrophysiological traces in panels a-c. The asterisk for cell a) indicates that these coordinates were flipped to the right half of the cerebellum. Scale bar = 50 microns. e (iii) Categorical grouping of complex spike phenotypes for internal versus caudal axonal projections. N = 17 cells from 17 fish. (f) Morphed Purkinje cell axonal morphologies from single-cell labelling across fish (N = 50 cells) can be grouped into two populations based on axonal projection (as for e iii). N = 27 cells with internal axons, N = 23 cells with caudal axons.