Fig. S6

Fig. S6

Brainwide Responses to vHb ChR Stimulation, Related to Figure 6

(A) Max-projection of confocal stack of 15 dpf Tg(ppp1r14ab:Gal4-VP16; UAS:ChR2-mCherry;elavl3:h2b-GCaMP6s) (scale bar = 150μm; yellow box demarcates region shown in Figure 6B). (B) The speed of ChR+ fish (blue, n = 9) and ChR– clutch mates (black, n = 16) in response to BC (left) followed by recovery in a visually distinct tank and then two minutes of 460 nm light pulses (20 Hz; 5 ms duration; 1 mW/mm²). ChR+ fish showed a significantly lower movement rate than ChR– fish during the stimulation period (p = 0.0184). (C) Outward photocurrents in an in vivo voltage-clamped mCherry+ vHb neuron from a Tg(dao:Gal4-VP16; UAS:NpHR-mCherry) fish (left) and mCherry– neuron from the same fish (right). Photocurrents were evoked by 2 s of 560 nm continuous light (yellow bars; average response indicated by solid black line; n = 3 pulses for both NpHR+ and NpHR–). (D) The percentage of cells in each fish that showed a significant excitatory (upper) or inhibitory (lower) response in the two seconds following stimulation in ChR+ fish (blue, n = 6) and ChR- fish (black, n = 4) in several regions both ipsilateral to stimulation (left) and contralateral (right). (E) The percentage of cells significantly responding 8-10 s post-stimulation. Error bars and shaded regions are s.e.m. All statistical tests are Student’s t tests unless otherwise indicated (^∗p < 0.05; ^∗∗p < 0.01; ^∗∗∗p < 0.001).