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Fig 4
Transected RGC axons grow to ipsilateral and contralateral tecta.
(A-L) Examples of RGC axonal tracings of Tg(isl2b:GFP) larvae at 72 hpt that were uninjured (A-D), received only a left optic nerve (ON) transection (E-H) or had both ON transected (I-L). Axons were traced by injecting the lipophilic dyes DiI and DiD into the left and right retina, respectively. RGC axons of uninjured nerves project to only contralateral tecta, while transected RGC axons grow to both ipsilateral and contralateral tecta. Asterisks indicate melanophores on the skin. Dashed lines outline dye fluorescence from the injected eye. Scale bar = 50 μm.
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