ZFIN Image: Sharma et al., 2019, Figure 1

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Figure Caption

Figure 1

Mindbomb1 is required for zebrafish spinal cord morphogenesis. (a,b) Morpholino knock-down of mib1 disrupts apical aPKC enrichment and neuro-epithelial morphology (outlined by F-actin staining) in 15/19 embryos. (c) A similar disruption of apico-basal polarity is observed in mib1 mutants (n = 14). (d,e) mib1 mutants fail to establish polarized Pard3 localization (e, n = 12). (f,g) No polarized Crumbs enrichment is observed in mib1 mutants (g, n = 17). (h,i) The apical localization of the tight junction component ZO-1 is disrupted in mib1 mutants (i, n = 16). (j,k) Centrosomes fail to move towards the neural tube midline in mib1 mutants (k, n = 16). (a–c,f,g) 30 somites stage. d,e, 18 somites stage, (h–k) 22 somites stage. All images are dorsal views of the anterior spinal cord, anterior left. Scalebars: 20 µm.

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